Listeria detection · rapid screening
Listeria monocytogenes LAMP Test Kit – isothermal detection in about one hour
The Listeria monocytogenes LAMP Test Kit detects L. monocytogenes DNA by loop-mediated isothermal amplification after enrichment. The reaction runs at one temperature in a heat block or PCR instrument and is read by colour change.
- Detection limit 8 CFU per test
- Visual colour read-out, no real-time PCR needed
- 24 tests per box · COLO code PRO7411
How the Listeria monocytogenes LAMP Test Kit works
After enrichment, DNA is released by heating the culture. The lyophilised reaction tubes contain primers specific for L. monocytogenes; at about 63 °C the target is amplified within 50 minutes and positive samples turn fluorescent green.
Reading the result
| Result | Reading |
| Positive | fluorescent green |
| Negative | light orange |
The test detects DNA and cannot distinguish live and dead cells; positive results are confirmed by the culture method.
Technical data
| Sample preparation | Release DNA by lysis at 99 °C for 10 min |
| Reaction | Lyophilised tubes reconstituted with 20 µL solution; controls with 80 µL ultrapure water |
| Amplification | 63 °C (60–65 °C), 50 min |
| Detection limit | 8 CFU per test |
| Storage | 2–8 °C |
| Shelf life | 12 months |
Applications and practical notes
The kit is used for rapid screening of enriched samples for L. monocytogenes; positive results are confirmed according to ISO 11290-1. Typical applications include:
- ready-to-eat food producers
- dairy and cheese producers
- fish and meat processing
- environmental monitoring programmes
Working with Listeria monocytogenes LAMP Test Kit: Work in separate areas for sample preparation and amplification, close the tubes before incubation and run the positive and negative controls with each series.
Good practice with rapid tests: store the kit as stated on the label, let the components reach room temperature before use and use each item only once. Record the lot number and the result, and confirm positive results according to the reference method when this is required.
About the Salmonella culture method: the reference method for Salmonella in the food chain, ISO 6579-1, has four steps: non-selective pre-enrichment, selective enrichment in two broths, plating on two selective agars and confirmation of typical colonies. The FDA BAM Chapter 5 follows the same principle with media adapted to different food types. Rapid tests and identification kits shorten the time to result, but positive screening results are usually confirmed by the culture method. COLO.Science offers products for every step, so that one supplier covers the whole procedure.
Complete sets from one supplier: COLO.Science supplies culture media, supplements, rapid tests, identification kits and QC strains as complete sets for the method you follow. Send us your sample types and standards, and we will prepare an offer with the matching products and documentation.
Listeria monocytogenes LAMP Test Kit is supplied by COLO.Science in the pack sizes listed under Ordering information. For larger quantities, regular deliveries or method-specific sets that include Listeria monocytogenes LAMP Test Kit, please send us a request. With every delivery of Listeria monocytogenes LAMP Test Kit you receive the lot number and expiry date, and on request we send the available documentation for Listeria monocytogenes LAMP Test Kit.
Where the Listeria monocytogenes LAMP Test Kit fits in the workflow
- Enrichment – Half-Fraser Broth or another Listeria enrichment
- Rapid screening – detection plate, LAMP or real-time PCR
- Confirmation – chromogenic Listeria agar, TSYEA, identification kit and latex
- Report – negative results reported quickly; positives confirmed
Ordering information
| Product | COLO code | Pack |
|---|---|---|
| Listeria monocytogenes LAMP Test Kit | PRO7411 | 24 tests |
| Listeria monocytogenes qPCR Detection Kit | PRO7394 | 48 tests |
Product notice: preparation, storage conditions and shelf life are given on the label. Technical data on this page are for orientation; the label and the lot documentation apply. B2B prices are quoted net, excluding VAT.
Sales and technical support
COLO LabExperts
Polje ob Sotli 4, SI-3255, Slovenia
Quotations and technical support for culture media and microbiology products across our international sales territory. B2B prices are net, excluding VAT.
COLO.SCIENCE | MICROBIOLOGY SUPPORT
Need the complete set for your method?
Send the sample type, target organism and the standard you follow. COLO.Science will put together the media, supplements, QC strains and confirmation tests for the whole procedure.
