Listeria detection · media supplement
LB2 Supplement – second enrichment step
Selective supplement for preparing LB2, the second enrichment broth for Listeria monocytogenes, from Listeria Enrichment Broth Base. One vial completes 200 mL.
- Selective supplement for Listeria Enrichment Broth Base
- Lyophilised, dissolved before use
- 10 vials per box · COLO code PRO7675
How the LB2 Supplement is used
The lyophilised supplement contains nalidixic acid and a higher amount of acriflavine than LB1, which increases the selectivity in the second step.
Technical data
| Composition per vial | nalidixic acid 4.0 mg, acriflavine hydrochloride 5.0 mg |
| Use | Dissolve in 1 mL sterile saline and add to 200 mL of Listeria Enrichment Broth Base |
| Storage | 2–8 °C |
| Shelf life | 2 years |
Applications and practical notes
LB2 is the second enrichment broth in national Listeria methods; the reference method ISO 11290-1 uses Half-Fraser and Fraser Broth. Typical applications include:
- ready-to-eat foods and delicatessen
- milk and dairy products
- fish and seafood
- environmental samples from food production
Working with LB2 Supplement: Dissolve the vial completely, add it only to the cooled sterile base and mix gently but thoroughly before dispensing or pouring. Keep unopened vials refrigerated.
Good practice with media and supplements: keep the bottle tightly closed in a cool, dry place, weigh the powder quickly to avoid moisture uptake, and use fresh distilled or deionised water. Check the pH of each new batch after preparation and before use, and label prepared media with the date. Performance of each lot can be verified with reference strains according to ISO 11133, the standard for the preparation, production, storage and performance testing of culture media.
About the Listeria culture method: the reference method for Listeria monocytogenes in the food chain, ISO 11290-1, uses primary enrichment in Half-Fraser Broth, secondary enrichment in Fraser Broth, plating on a chromogenic agar of the Ottaviani-Agosti type and a second selective agar, and confirmation of typical colonies. The FDA BAM Chapter 10 uses Buffered Listeria Enrichment Broth instead. COLO.Science offers media, supplements, rapid tests, identification kits and QC strains for every step.
Complete sets from one supplier: COLO.Science supplies culture media, supplements, rapid tests, identification kits and QC strains as complete sets for the method you follow. Send us your sample types and standards, and we will prepare an offer with the matching products and documentation.
LB2 Supplement is supplied by COLO.Science in the pack sizes listed under Ordering information. For larger quantities, regular deliveries or method-specific sets that include LB2 Supplement, please send us a request. With every delivery of LB2 Supplement you receive the lot number and expiry date, and on request we send the available documentation for LB2 Supplement.
Where LB2 fits in the Listeria workflow
- Primary enrichment – Half-Fraser Broth
- Secondary enrichment – Fraser Broth
- Selective isolation – chromogenic Listeria agar (Ottaviani-Agosti type) plus PALCAM or Oxford Agar
- Confirmation – subculture on TSYEA, biochemical identification and latex
Ordering information
| Product | COLO code | Pack |
|---|---|---|
| Listeria Enrichment Broth (LB2) Supplement | PRO7675 | 10 vials |
| Listeria Enrichment Broth Base, granulated | PRO7630 | 250 g |
Product notice: preparation, storage conditions and shelf life are given on the label. Technical data on this page are for orientation; the label and the lot documentation apply. B2B prices are quoted net, excluding VAT.
Sales and technical support
COLO LabExperts
Polje ob Sotli 4, SI-3255, Slovenia
Quotations and technical support for culture media and microbiology products across our international sales territory. B2B prices are net, excluding VAT.
COLO.SCIENCE | MICROBIOLOGY SUPPORT
Need the complete set for your method?
Send the sample type, target organism and the standard you follow. COLO.Science will put together the media, supplements, QC strains and confirmation tests for the whole procedure.
