COLO.Science · Technical Specification
FLUO-UP950 · Research Grade Fluorescence Microscope · Technical Specification
| Parameter | Specification / Value |
|---|---|
| Microscope type | FLUO-UP950 research grade fluorescence microscope, upright, full motorized |
| Optical system | Infinite optical system |
| Main body | Research-grade robust metal microscope body with integrated electric control concept |
| Eyepieces | SW10× / 25 |
| Viewing head | Tilting trinocular viewing head, Eyepiece/Port: 100/0, 20/80, 0/100; inclined at 0–35° |
| Interpupillary distance | 47–78 mm |
| C-mount | 1× C-mount |
| Illumination | 10W LED illumination with coded, adjustable constant-intensity light control |
| LED control features | ECO mode goes to standby after 15 minutes of inactivity; LED source control is integrated in the software |
| Nosepiece | Motorized coded sextuple nosepiece with Active Light Manager support |
| Objective 1 | S-APO PH 10×, NA 0.25, WD 16 mm |
| Objective 2 | S-APO FL 20×, NA 0.50, WD 2.7 mm |
| Objective 3 | S-APO FL 40×, NA 0.75, WD 1.4 mm |
| Objective 4 | S-APO FL 100× oil, NA 1.25, WD 0.18 mm |
| Additional magnification | 1.6× additional magnification for all contrast methods |
| Condenser | Universal condenser NA 0.9 for objective magnification 1–100× |
| Condenser inserts | Aplanatic-achromatic condenser, 5-position phase contrast including 10×, 20× and 40× PH inserts |
| DIC accessories | DIC condenser including 20×–40× DIC insert, polarizer and analyzer |
| Focusing | Motorized coaxial coarse and fine adjustment |
| Fine division | 1 μm |
| Focusing range | 35 mm |
| Stage | Motorized stage size 305 × 178.5 mm, XY travel 125 × 75 mm, fine 0.1 mm, four-slide holder, XYZ control joystick |
| Motorized movement | Micro electric control platform with controlled movement in X, Y and Z directions |
| Integrated control concept | Single USB cable connection from computer to built-in controller for integrated electric control |
| Z-axis design | High-precision motor screw structure for nano-scale Z-axis layer cutting scanning |
| Parameter | Specification / Value |
|---|---|
| Observation methods | Bright field and fluorescence; platform also supports phase contrast and DIC accessory workflow |
| Filter turntable | Motorized 6-position fluorescent filter turntable, coded |
| Excitation source | Coded LED fluorescence light source: 385 nm, 470 nm, 565 nm, 625 nm |
| Multi-band filter excitation | 385, 475, 555 and 630 nm |
| UV band | 435/30; suitable examples include DAPI, Hoechst 33342, Hoechst 33258, Alexa Fluor 350, Alexa Fluor 405, Indo-1, eBFP/BFP, eGFP (wt), True Blue |
| B band | 514/30; suitable examples include FM1-43, Cy2, eGFP, NBD, MitoTracker Green, Alexa Fluor 488, BCECF, Calcein, DiO, SNAFL, YO-Pro-1, Nissl, LysoSensor Green, mHoneydew, FITC / Fluorescein, Kaede, PerCP, YoYo-1, FuraRed |
| G band | 592/25; suitable examples include TRITC, 7-AAD, Cy3, tdTomato, Alexa Fluor 546, Alexa Fluor 555, DsRed, mOrange, TagRFP, SNARF, DyLight 549, Spectrum Orange |
| R band | 709/100; suitable examples include Alexa Fluor 633, Alexa Fluor 647, Cy5, DRAQ5, ToTo-3, ATTO-655, MitoTracker DeepRed, APC, ATTO-647N |
| Turntable application note | The fluorescence turntable can be equipped with up to 6 color filter sets for simultaneous imaging of multiple stained specimens; one-click switching improves efficiency and reduces dye quenching |
| Fluorescence filter technology | High-transmittance, sharp-cutoff fluorescence color filter sets with sub-ripple elimination coating technology for high sensitivity and improved signal-to-noise ratio |
| Optional fluorescence source choices | Mercury light source, metal halide lamp, or LED light source depending on final configuration |
| Mercury source note | Cost-effective, wide spectral range, simple to operate, effective life of 200 hours |
| Metal halide note | Brighter broad-spectrum source with use time up to 2,000 hours |
| LED source note | Cold light source with service life up to 5,000 hours and low damage to cells; UP950 series can be installed with 4 LED light sources |
| Camera | 2/3" sensor, 5 MP, pixel size 3.45 μm, USB 3.0, resolution 2448 × 2048, 35 fps @ 2448 × 2048, dynamic range 4800:1, C-mount |
| Parameter | Specification / Value |
|---|---|
| Touch control screen | Front-mounted touch control screen integrated with the microscope, space-saving and programmable |
| Touch control functions | Three-axis coordinate display, speed gear display, objective electric switching, dual hole orientation switching, position memory and return, relative coordinate display, objective parfocal compensation, temporary upper limit setting, platform escape and recovery, day/night mode |
| Software platform | COLO Workstation: computer controlled plus motorized microscope imaging system software |
| Software functions | Integrated control for microscope, camera, motorized platform and components; motorized control, autofocus, sequence scanning, fixed-point scanning, surface fitting scanning, full-field focusing scanning, measurement, synthesis and data recording |
| Additional software modules | Multichannel fluorescence, manual panorama, manual extended focus, plus COLO NOMIS basic imaging analysis software |
| Processor | Intel® CPU with 6 cores, 3 GHz clock frequency, e.g. Intel® Core® i5-12500 |
| Memory | 16 GB RAM |
| PCI Express | 1 × PCI Express Generation 3 ×16 |
| Graphics | Minimum 1920 × 1200 resolution, 32-bit true color, DirectX® 11.0 or higher, e.g. integrated Intel® UHD Graphics 630 or NVIDIA T1000 8 GB |
| Storage | 1 × 512 GB SSD NVMe |
| USB ports | 2 × USB 2.0 and 2 × USB 3.0 |
| Monitor | 27" TFT display, vertical resolution 1200 pixels |
| Operating system | Microsoft® Windows® 10 x64 Professional |
| Power supply | 220V, 50/60Hz |
| Configured microscope set | FLUO-UP950 microscope body, trinocular viewing head, SW10×/25 eyepieces, motorized coded sextuple nosepiece, listed S-APO objective set, motorized stage, motorized focus system, universal condenser, coded 10W LED illumination and fluorescence filter turntable system |
| Imaging bundle | 5 MP USB 3.0 C-mount camera with 2448 × 2048 resolution and COLO Workstation / COLO NOMIS software environment |
| Workstation bundle | Recommended PC specification with Intel® 6-core CPU, 16 GB RAM, 512 GB SSD NVMe, 27" TFT monitor and Windows 10 x64 Professional |
| Configuration note | The catalog describes mercury, metal halide and LED fluorescence source options. Final supplied configuration should follow quotation and order confirmation. |