COLO.Science · Technical Specification
FLUO-UP900
Research Grade Fluorescence Microscope · Full Motorized Upright System
Research-grade upright fluorescence microscope with motorized stage movement, coded sextuple nosepiece, full Köhler illumination, touchscreen and joystick control, and integrated computer-based imaging workflow. Designed for advanced microscopy in bright field, phase contrast, DIC, dark field, and fluorescence applications, with workstation software for autofocus, scanning, data acquisition, and image processing.
UIS2 optical system
Full motorized
Motorized X/Y/Z stage
Sextuple coded nosepiece
Full Köhler illumination
Trinocular head
BF / DF / DIC / FL
MG100 LED fluorescence
MC100 controller
UNISON-5PA camera
FISH capable
COLO Workstation
Product
FLUO-UP900 · fluorescence microscope
Address (HQ)
Polje ob Sotli 4
SI-3255, Slovenia
SI-3255, Slovenia
Contact
FLUO-UP900 · Research Grade Fluorescence Microscope · Technical Specification
Document ID: COL-TEC-FLUO-UP900-EN
Version: Responsive layout
Informational technical sheet · Non-binding · Controlled electronic document
Version: Responsive layout
Informational technical sheet · Non-binding · Controlled electronic document
FLUO-UP900 Research Grade Fluorescence Microscope Full motorized operation · trinocular optics · fluorescence imaging workstation
Upright microscope
Motorized nosepiece
Motorized X/Y/Z
76 × 54 mm travel
1 μm fine focus
6 objective positions
26.5 mm FOV
4 turret fluorescence
2-channel light gate
50,000 h LED source
5 MP camera
27" workstation
Technical specifications – Microscope platform
| Parameter | Specification / Value |
|---|---|
| System type | Research grade fluorescence microscope, upright, full motorized |
| Optical system | UIS2 optical system |
| Microscope body | Microscope frame for transmitted and reflected microscopy |
| Viewing head | Widefield trinocular viewing head, 30° inclined, beamsplitter 0/100 and 50/50 |
| Interpupillary distance | 45–78 mm |
| Eyepiece tube diameter | Φ30 mm |
| Field of view | 26.5 mm |
| Eyepieces | Super widefield high eyepoint eyepieces SWH10X / 25 mm |
| Nosepiece | Sextuple coded nosepiece, 6 objective positions, RMS thread, with DIC slot |
| Objective 1 | PLAN10XPH infinite plan achromatic phase contrast objective, 10×, NA 0.25, WD 10.8 mm |
| Objective 2 | PLAN 20X infinite plan achromatic objective, 20×, NA 0.40, WD 1.5 mm |
| Objective 3 | PLAN 40X infinite plan achromatic objective, 40×, NA 0.65, WD 15 mm |
| Objective 4 | PLAN 100X infinite plan achromatic objective, 100×, NA 1.25, WD 0.21 mm |
| Stage | Mechanical stage 235 × 150 mm, Gorilla glass insert, right handle, travel range 76 × 54 mm |
| Motorized movement | Motorized X, Y and Z movement, joystick and touchscreen controlled |
| Focus | Vertical stage movement 25 mm, coarse adjustment limit stopper, torque adjustment, variable stage mounting position, high sensitivity fine focus |
| Minimum focusing increment | 1 μm |
| Condenser | Universal condenser with 8 positions: 1 BF, 3 phase contrast, 3 DIC, 1 dark field |
| Phase ring | Phase contrast ring for 10× objective |
| Top lens | NA 0.9 top lens for universal condenser |
| Transmitted illumination | Built-in Köhler illumination, light preset switch, light intensity manager switch, high color reproductivity LED transmitted light source |
| Transmitted LED section detail | Detailed technical section states 18W LED transmitted light source |
| Control interface | Touchscreen and joystick operation, programmable control, position memory and return, relative coordinate display, parfocal compensation, temporary upper limit setting, platform escape and recovery, day/night display mode |
Technical specifications – Fluorescence module & digital imaging
| Parameter | Specification / Value |
|---|---|
| Epi-illuminator methods | Bright field, dark field, fluorescence |
| Aperture diaphragm | Yes, centerable |
| Field diaphragm | Yes, centerable |
| Polarized slot | Yes |
| Filter slot | Yes |
| Auxiliary focus | None |
| Turret position | 4 |
| Protection | Fluorescent protection plate |
| Fluorescence source | MG100 ultra-high-power long-life LED fluorescent solid light source, suitable for FISH |
| Light-emitting unit | Critical solid-state light-emitting diode |
| Wavelength range | 350–390 nm, 410–740 nm |
| Electronic light gate | 2 independent channels |
| Dimming | 0–100% stepless continuous |
| Subdivision accuracy | 1% gradual |
| Wick life | 50,000 hours |
| Light decay period | 30% decay after 25,000 hours |
| Refresh frequency | 100 MHz |
| High-speed photography support | Up to 16,000 FPS |
| Fluorescence source power supply | 100 W |
| Cooling system | Active circulating heat pipe |
| Light controller | MC100 controller with keyboard and LCD display |
| Controller functions | 1% brightness step adjustment, electronic light shutter, brightness and wavelength display, PC control for pulse, strobe and duty cycle adjustment |
| Computer control | USB external trigger control |
| Filter cube | GR/OR bicolor excitation filter cube U-FF-GR/OR, EX 480–560 nm, dichroic 517/613 nm, emission 523–617 nm, suitable for FISH |
| Multi-band filter | Excitation: 385 / 475 / 555 / 630 nm; Emission bands for DAPI/Hoechst, Cy2/eGFP/Alexa Fluor 488/FITC, TRITC/Cy3/tdTomato/Alexa Fluor 546–555/DsRed/mOrange, and Alexa Fluor 633/647/Cy5 families |
| Turntable excitation module | Fluorescence turntable can be equipped with up to 6 color filter sets for multistained specimen imaging |
| C-mount adapter | 0.5× C-mount adapter for digital camera |
| Digital camera | UNISON-5PA digital camera, 5.0 MP, IMX264(C, GS), 2/3" CMOS, sensor size 8.45 × 7.07 mm, pixel size 3.45 × 3.45 μm |
| Camera frame rates | 35 fps @ 2448 × 2048, 50 fps @ 1224 × 1024 |
| Image software | Supplied with image processing software |
Technical specifications – COLO Workstation
| Parameter | Specification / Value |
|---|---|
| Software platform | COLO Workstation: computer controlled motorized microscope imaging system software with COLO NOMIS basic imaging analysis software |
| Software functions | Motorized control, autofocus, sequence scanning, fixed-point scanning, surface fitting scanning, full-field focusing scanning, measurement, synthesis, data recording, multichannel fluorescence, manual panorama, manual extended focus |
| Processor | Intel® CPU with 6 cores, 3 GHz clock frequency, e.g. Intel® Core® i5-12500 |
| Memory | 16 GB RAM |
| PCI Express | 1 × PCI Express Generation 3 ×16 |
| Graphics | Minimum 1920 × 1200 resolution, 32-bit true color, DirectX® 11.0 or higher, e.g. Intel® UHD Graphics 630 or NVIDIA T1000 8 GB |
| Storage | 1 × 512 GB SSD NVMe |
| USB ports | 2 × USB 2.0 and 2 × USB 3.0 |
| Monitor | 27" TFT display, vertical resolution 1200 pixels |
| Operating system | Microsoft® Windows® 10 x64 Professional |
| Connection concept | Single USB cable connection from PC to built-in controller for integrated electric control |
Key Features of FLUO-UP900
Motorization & Precision
- Full motorized microscope with electric platform and electric objective lens conversion
- Motorized X, Y and Z motion with joystick and touchscreen control
- 25 mm vertical movement and 1 μm minimum focusing increment
- Position memory, return, escape and recovery functions
- Parfocal compensation and temporary upper limit setting
- Precise integrated control for observation, acquisition and image processing
Optics & Fluorescence
- UIS2 optical system with full Köhler illumination
- Trinocular head with 26.5 mm field of view
- Bright field, dark field, fluorescence, phase contrast and DIC capability
- MG100 long-life fluorescence source with 2 independent light gates
- Up to 6 color filter sets on the fluorescence turntable
- High-sensitivity fluorescence detection with reduced excitation burden on cells
Software & Imaging Workflow
- Touch control screen with intuitive programmable interface
- COLO Workstation software for autofocus and advanced scanning workflows
- UNISON-5PA 5 MP camera with image processing software
- Manual panorama and manual extended focus support
- Integrated digital control of microscope, camera and platform
- Fast and efficient repeatable imaging setup restoration
Typical applications
- Advanced fluorescence microscopy in research laboratories
- Bright field and fluorescence image acquisition workflows
- Phase contrast and DIC observation in life science laboratories
- FISH-compatible fluorescence imaging
- Multichannel fluorescence observation of stained specimens
- Routine and research microscopy in universities and institutes
- Digital documentation, measurement and image synthesis
- Computer-controlled microscopy with repeatable automated positioning
Configured package in this specification
| Configured microscope set | FLUO-UP900 microscope body, widefield trinocular head, SWH10X/25 eyepieces, sextuple coded nosepiece, listed objective set, motorized stage and focus system, universal condenser, transmitted light system, fluorescence epi-illumination module, MG100 fluorescence source, MC100 light controller, C-mount adapter and UNISON-5PA camera |
| Workstation bundle | COLO Workstation software environment with recommended PC specification, 27" monitor and Windows 10 x64 Professional operating platform |
| Configuration note | Catalog overview also mentions optional fluorescent light source choices such as mercury, metal halide and LED-based configurations. Final supplied configuration should follow quotation and order confirmation. |
Configuration note – FLUO-UP900
The catalog combines series overview information and detailed configuration pages. This technical sheet follows the detailed FLUO-UP900 configuration pages for the trinocular head, objective set, motorized stage, condenser, MG100 fluorescence source, MC100 controller, camera and workstation. Where the catalog uses broader series wording, final supply scope should be confirmed in the official quotation.
The catalog combines series overview information and detailed configuration pages. This technical sheet follows the detailed FLUO-UP900 configuration pages for the trinocular head, objective set, motorized stage, condenser, MG100 fluorescence source, MC100 controller, camera and workstation. Where the catalog uses broader series wording, final supply scope should be confirmed in the official quotation.
Frequently asked questions – FLUO-UP900
Is the microscope motorized? Yes, the system is described as full motorized, with motorized stage movement and electric objective conversion.
Which observation modes are supported? The platform supports bright field, dark field, fluorescence, phase contrast and DIC-based configurations.
Can the system be used for FISH? Yes, the MG100 fluorescence source is explicitly noted as suitable for FISH.
How many filter sets can be installed? The fluorescence turntable can be equipped with up to 6 color filter sets.
Is digital imaging included? Yes, the configured system includes a 5 MP UNISON-5PA camera and image processing software.
Can it be computer-controlled? Yes, the microscope integrates with COLO Workstation software for autofocus, scanning and digital workflow control.
Is the microscope motorized? Yes, the system is described as full motorized, with motorized stage movement and electric objective conversion.
Which observation modes are supported? The platform supports bright field, dark field, fluorescence, phase contrast and DIC-based configurations.
Can the system be used for FISH? Yes, the MG100 fluorescence source is explicitly noted as suitable for FISH.
How many filter sets can be installed? The fluorescence turntable can be equipped with up to 6 color filter sets.
Is digital imaging included? Yes, the configured system includes a 5 MP UNISON-5PA camera and image processing software.
Can it be computer-controlled? Yes, the microscope integrates with COLO Workstation software for autofocus, scanning and digital workflow control.
Please note: If this fluorescence microscope configuration does not exactly match your application, contact our team. We can help with microscope configuration, digital imaging workflow, fluorescence filter selection and application-specific setup. Specifications subject to change without notice.
Contact & Support
COLO LabExperts
Polje ob Sotli 4
SI-3255, Slovenia
Support note: Contact us for fluorescence microscope configuration, camera integration, filter selection and application-specific microscopy solutions.
Quick contact
Manufacturer & configuration notice:
Specifications in this document are based on the uploaded FLUO-UP900 catalog and arranged into the COLO.Science technical sheet format.
For procurement and tender purposes, the only valid and binding specification is the official COLO.Science quotation and the accompanying technical specification that is engineering-approved.
Specifications in this document are based on the uploaded FLUO-UP900 catalog and arranged into the COLO.Science technical sheet format.
For procurement and tender purposes, the only valid and binding specification is the official COLO.Science quotation and the accompanying technical specification that is engineering-approved.