COLO.Science · Technical Specification
FLUO500
Fluorescence Inverted Microscope with Phase Contrast · Binocular / Trinocular · Live Cell Imaging
FLUO500 is a fluorescence inverted microscope family designed for live cell imaging, phase contrast observation and routine fluorescence applications.
The system combines infinity-corrected long working distance optics, a reversed 5-position nosepiece, ELWD condenser, flexible stage configurations and fluorescence filter sets for research, medical, biological and industrial laboratories.
Inverted microscope
Phase contrast
Fluorescence imaging
5-objective nosepiece
LWD objectives
ELWD condenser
100W mercury lamp
100-240V supply
Live cell imaging
Model
FLUO500 · fluorescence inverted microscope family
Address (HQ)
Polje ob Sotli 4
SI-3255, Slovenia
SI-3255, Slovenia
Contact
FLUO500 · Fluorescence Inverted Microscope with Phase Contrast · Technical Specification
Document ID: COL-TEC-FLUO500-EN
Version: Responsive layout
Informational technical sheet · Non-binding · Controlled electronic document
Version: Responsive layout
Informational technical sheet · Non-binding · Controlled electronic document
FLUO500 Fluorescence Inverted Microscope with Phase Contrast Binocular / trinocular · infinity LWD optics · fluorescence + phase contrast
Live cell imaging
Infinity optics
Anti-fungus optics
Anti-reflection coated
Long working distance
Reversed nosepiece
Coaxial focus
Phase contrast
Fluorescence filters
Product family and variants
| Variant | Typical configuration note |
|---|---|
| FLUO500T | Extended EWF 10×/20 mm eyepieces with Ø30.0 mm tubes; catalog family version with 45° inclined viewing geometry. |
| FLUO502T | Extended EWF 10×/20 mm eyepieces with Ø23.2 mm tubes; Ø30.0 mm optional; catalog family version with 30° inclined viewing geometry. |
| Family note | Exact delivered head, stage, filters, objectives and camera adapters depend on final selected configuration. |
Technical specifications
| Product type | Fluorescence inverted microscope with phase contrast |
| Observation mode | Bright field, phase contrast and fluorescence observation |
| Microscope format | Inverted microscope for live cell imaging and cell culture applications |
| Eyepieces | Extended EWF 10×/20 mm; eyepieces can be secured to the tubes |
| Tube diameter | Ø30.0 mm for FLUO500T; Ø23.2 mm for FLUO502T; Ø30.0 mm optional on FLUO502T |
| Viewing head | Binocular / trinocular Seidentopf type head with diopter adjustment; 45° or 30° inclined depending on version; interpupillary distance 52–75 mm |
| Photo port | Ø30 mm photo port on trinocular head |
| Optional ergonomic head | Tilting head available with 0:100 / 50:50 beam split configuration |
| Nosepiece | Revolving and reversed nosepiece for 5 objectives |
| Optical treatment | All optics are anti-fungus treated and anti-reflection coated for maximum light throughput |
| Bright field objectives | Infinity Plan Achromatic LWD objectives: 4×/0.13 (optional), 10×/0.25, 20×/0.40, 40×/0.60 (optional) |
| Phase contrast objectives | Infinity Plan Achromatic LWD phase contrast objectives: 4×/0.13 (optional), 10×/0.25 (optional), 20×/0.40, 40×/0.60 (optional) |
| Fluorescence filters | B / G / R / Y / UV / V fluorescence filter sets |
| Stage | Attachable mechanical stage; optional ceramic mechanical stage 180 × 155 mm with integrated 80 × 50 mm mechanical stage |
| Bright field condenser | ELWD condenser, NA 0.30, LWD 72 mm; approx. 150 mm after taking down the condenser |
| Focusing system | Coaxial coarse and fine adjustment; 100 graduations; 1 μm per graduation; 100 μm per rotation; total travel approx. 14 mm; rack stop and friction control |
| Illumination | 100 W mercury lamp; adjustable halogen illumination available; internal 100–240 V power supply |
| Optical performance note | Large apertures support high-resolution imaging close to the theoretical diffraction limit of the optics |
| Packaging | Supplied with power cord, dust cover, spare fuse and user manual; packed in foam and carton |
Highlights
Optical system
- Inverted microscope configuration for live cell work
- Infinity-corrected long working distance objectives
- Anti-fungus treated and anti-reflection coated optics
- Bright field and phase contrast observation capability
- Fluorescence filter sets for multiple excitation ranges
- 5-position reversed nosepiece for flexible objective setup
Mechanical and illumination features
- Coaxial coarse and fine focusing with rack stop
- Interpupillary adjustment 52–75 mm
- ELWD condenser for cell culture vessels and live samples
- 100 W mercury lamp fluorescence illumination
- Internal 100–240 V power supply
- Optional stage, head and camera adaptation accessories
Typical applications
- Live cell imaging
- Cell culture observation
- Medical and biological research
- Pharmacy and clinical diagnosis
- Agriculture and animal husbandry studies
- Forensic and criminal investigation work
- Environmental protection engineering
- Preventive medicine and teaching laboratories
Configurable options and accessories
| Camera / imaging adapters | Adapters for Nikon DSLR, Canon DSLR, 0.5× C-mount, 0.25× C-mount and X C-mount are available depending on configuration |
| Stage / sample support options | Attachable or integrated mechanical stages, specimen holders, microplate holder and microscope slide holders depending on selected package |
| Phase contrast accessories | Phase contrast sliders and corresponding phase contrast objectives available for selected magnifications |
| Fluorescence accessories | Multi-functional filter turret and blue / green / red / violet / ultraviolet excitation filter sets according to final requested setup |
| Additional illumination accessories | Transmitted illumination, UV shield, filters with slider, upper LED light and related lamp house accessories may be available depending on version |
What's in the box
| Standard supply | FLUO500 microscope main unit, selected head configuration, power cord, dust cover, spare fuse, user manual |
| Configuration-dependent items | Objectives, phase contrast accessories, fluorescence filter sets, stage components, imaging adapters and illumination modules according to ordered version |
*Delivered configuration should always be confirmed in the quotation.
FAQ – FLUO500
Is FLUO500 suitable for live cell imaging? Yes. The catalog specifically presents the inverted microscope platform as suitable for live cell imaging.
Does it support phase contrast? Yes. Phase contrast is a core feature of the FLUO500 family, with dedicated phase contrast objectives and accessories depending on configuration.
Can it be used for fluorescence imaging? Yes. The system supports fluorescence observation with multiple filter sets including blue, green, red, yellow, UV and violet configurations.
Can I connect a camera? Yes. Trinocular versions provide a photo port, and DSLR / C-mount adapter options are available depending on the setup.
Are all options standard? No. The official catalog shows configuration-dependent equipment, so the final set of optics, filters, stages and adapters should be matched to the application.
Is FLUO500 suitable for live cell imaging? Yes. The catalog specifically presents the inverted microscope platform as suitable for live cell imaging.
Does it support phase contrast? Yes. Phase contrast is a core feature of the FLUO500 family, with dedicated phase contrast objectives and accessories depending on configuration.
Can it be used for fluorescence imaging? Yes. The system supports fluorescence observation with multiple filter sets including blue, green, red, yellow, UV and violet configurations.
Can I connect a camera? Yes. Trinocular versions provide a photo port, and DSLR / C-mount adapter options are available depending on the setup.
Are all options standard? No. The official catalog shows configuration-dependent equipment, so the final set of optics, filters, stages and adapters should be matched to the application.
Please note: If this microscope does not exactly match your application needs, or if a required option is not listed in the standard package, contact us. Our team can recommend a suitable FLUO500 configuration for fluorescence, phase contrast, camera integration and sample holder requirements. Technical specifications are subject to change without notice.
Contact & Support
COLO LabExperts
Polje ob Sotli 4
SI-3255, Slovenia
Support note: Contact our team for microscope configuration, fluorescence filter selection, camera adapter compatibility and application-specific recommendations.
Quick contact
Manufacturer & configuration notice:
Specifications in this document are based on the FLUO500 catalog family. COLO.Science reserves the right to change technical specifications without notice.
For procurement and tender purposes, the only valid and binding specification is the official COLO.Science quotation and the accompanying technical specification that is engineering-approved.
Specifications in this document are based on the FLUO500 catalog family. COLO.Science reserves the right to change technical specifications without notice.
For procurement and tender purposes, the only valid and binding specification is the official COLO.Science quotation and the accompanying technical specification that is engineering-approved.