COLO.Science · Technical Specification
FLUO-IN900
Scientific Research Inverted Fluorescence Microscope · Trinocular System
Research-grade inverted fluorescence microscope designed for advanced life science work with bright field, phase contrast, fluorescence, polarized light and DIC-ready workflow support. The FLUO-IN900 combines a trinocular observation head, long working distance semi-apochromatic phase objectives, Köhler LED transmitted illumination, a 100 W HBO fluorescence source and a six-position fluorescence attachment for versatile laboratory imaging and observation tasks.
Inverted microscope
Fluorescence
Bright field
Phase contrast
Polarized light
DIC ready
NIS infinite optics
Köhler LED transmitted
100 W HBO lamp
6-position turret
1.5x lens changer
1 µm precision
Product
FLUO-IN900 · inverted fluorescence microscope
Address (HQ)
Polje ob Sotli 4
SI-3255, Slovenia
SI-3255, Slovenia
Contact
FLUO-IN900 · Scientific Research Inverted Fluorescence Microscope · Technical Specification
Document ID: COL-TEC-FLUO-IN900-EN
Version: Responsive layout
Informational technical sheet · Non-binding · Controlled electronic document
Version: Responsive layout
Informational technical sheet · Non-binding · Controlled electronic document
FLUO-IN900 Scientific Research Inverted Fluorescence Microscope trinocular configuration · long working distance optics · fluorescence-ready research platform
Inverted
Trinocular head
45° tubes
47-78 mm IPD
340 × 230 mm stage
XY 130 × 85 mm
1 µm fine focus
NA 0.55 condenser
LED 10 W transmitted
100 W HBO fluorescence
Blue/green filters
100-240 V
Technical specifications - Microscope platform
| Parameter | Specification / Value |
|---|---|
| Microscope type | FLUO-IN900 scientific research inverted fluorescence microscope |
| Observation modes | Bright field, phase contrast, fluorescence, polarized light and DIC-ready configuration; the series description also positions the platform for advanced life science imaging workflows |
| Optical system | Unique NIS infinite optical system |
| Eyepieces | HWF 10× / 25 mm eyepieces |
| Viewing head | Trinocular head with 45° inclined tubes |
| Beam splitter positions | 100-0 / 20-80 / 0-100 |
| Interpupillary distance | 47-78 mm |
| Diopter adjustment | Adjustable on both eyepieces |
| Nosepiece | Reversed sextuple nosepiece on ball bearings |
| DIC compatibility | Optional DIC prisms for 10×, 20× and 40× objectives |
| Optical treatment | Objectives are anti-fungus treated and anti-reflection coated for high light throughput |
| Standard objective 1 | Infinity Plan Semi-Apochromatic Phase PH 10× / NA 0.30 / WD 10.2 mm / cover glass correction 1.2 mm |
| Standard objective 2 | Infinity Plan Semi-Apochromatic Phase PH 20× / NA 0.45 / WD 7.5-8.8 mm with collar / cover glass correction 1.2 mm |
| Standard objective 3 | Infinity Plan Semi-Apochromatic Phase PH 40× / NA 0.60 / WD 3-4.4 mm with collar / cover glass correction 1.2 mm |
| Stage | Mechanical stage 340 × 230 mm with coaxial X-Y movement 130 × 85 mm |
| Included stage inserts | Metal insert for Ø38 mm culture dish; metal insert for Ø54 mm dish or 76 × 26 mm slides; metal insert for multiwell plates (127.76 × 85.48 mm); metal insert for Terasaki plates (83.3 × 58.0 mm) |
| Focusing | Coaxial coarse and fine adjustment with 200 graduations |
| Fine focus precision | 1 µm; 0.2 mm per rotation |
| Total focus travel | Approximately 10 mm |
| Condenser | Rotating condenser, N.A. 0.55 with iris diaphragm |
| Condenser working distance | 26 mm |
| Phase annuli | For 10×/20×, 40× and 60× objectives |
| Condenser DIC compatibility | DIC prisms for 10×/20×/40× and 60× objectives (additional accessories required) |
| Filter holders | Three positions for Ø38 mm filters |
| Condenser arm | Tilting arm for easy stage access and placement of larger items such as flasks and Petri dishes |
| Lens changer | Integrated 1.5× lens changer for intermediate magnifications without refocusing |
Technical specifications - Illumination & fluorescence module
| Parameter | Specification / Value |
|---|---|
| Transmitted illumination | 10 W LED transmitted illumination with adjustable intensity |
| Power supply | Internal power supply, 100-240 V |
| Illumination style | Köhler LED transmitted illumination |
| Fluorescence light source | 100 W mercury vapor HBO lamp |
| Fluorescence attachment | Six-position fluorescence attachment with UV shield |
| Standard fluorescence filters | Blue and green interference Ø45 mm filters |
| Standard fluorescence filter set 1 | Blue excitation: EX 460-495 nm (BP), DM 505, EM 510 nm (LP) |
| Standard fluorescence filter set 2 | Green excitation: EX 510-550 nm (BP), DM 570, EM 575 nm (LP) |
| Optional filter set - blue | EX 460-490 nm (BP), DM 500 nm, EM 520 nm (LP), requires empty filter cube |
| Optional filter set - green | EX 510-550 nm (BP), DM 570 nm, EM 590 nm (LP), requires empty filter cube |
| Optional filter set - violet | EX 400-410 nm (BP), DM 455 nm, EM 460 nm (LP), requires empty filter cube |
| Optional filter set - ultra-violet | EX 330-385 nm (BP), DM 400 nm, EM 420 nm (LP), requires empty filter cube |
| Optional filter set - red | BP 620-650, dichroic mirror DM 660, barrier BA 670-750, requires empty filter cube |
| Additional fluorescence accessories | 100 W power supplier NFP-1, FL-ND6 filter and FL-ND25 filter are listed in the accessory range |
Technical specifications - Objectives, imaging & accessories
| Parameter | Specification / Value |
|---|---|
| Optional phase objective | 4× / NA 0.13 / WD 17.78 mm; requires phase annuli for 4× objective |
| Optional phase objective | 60× / NA 0.70 / WD 1.8-2.6 mm with collar / cover glass correction 0.1-1.3 mm |
| Optional fluorescent objective | 4× / NA 0.13 / WD 17 mm |
| Optional fluorescent objective | 10× / NA 0.30 / WD 7.3 mm / cover glass correction 1.2 mm |
| Optional fluorescent objective | 20× / NA 0.45 / WD 8 mm / cover glass correction 1.2 mm |
| Optional fluorescent objective | 40× / NA 0.60 / WD 3.6 mm / cover glass correction 1.3 mm |
| Optional fluorescent objective | 60× / NA 0.70 / WD 1.8-2.6 mm with collar / cover glass correction 0.1-1.3 mm |
| Optional Plan-Apo objective | 10× / NA 0.45 / WD 4.0 mm / cover glass correction 0.17 mm |
| Digital imaging option | 9.7" LCD screen integrated with 5 MP digital camera, Android OS, HDMI, USB, SD card, Wi-Fi and mouse control |
| C-mount accessory | 1× magnification C-mount for C-mount camera |
| C-mount relay option | 0.50× relay objective for 1/2" C-mount camera |
| C-mount relay option | 0.35× relay objective for 1/3" C-mount camera |
| C-mount relay option | 0.63× relay objective for 2/3" C-mount camera |
| SLR photography adapter | Universal Ø23.2 mm tube adapter with built-in 2× lens for APS-C SLR camera; T2 ring required |
| Camera T2 rings | T2 ring for Nikon D SLR digital camera; T2 ring for Canon EOS SLR digital camera |
| Additional holders | Universal cell culture holder and rotating magnification changer with 1.5× lens are listed as accessories |
Key Features of FLUO-IN900
Research Inverted Platform
- Scientific research inverted microscope for advanced life science observation
- Supports bright field, fluorescence, phase contrast, polarized light and DIC-ready workflows
- Trinocular head with flexible light path positions for viewing and imaging
- Long working distance semi-apochromatic phase objectives for culture observation
- Designed to reduce operator fatigue during longer observation sessions
- Modular structure allows flexible imaging configuration
Optics & Fluorescence
- NIS infinite optical system for improved flatness and image quality
- Köhler 10 W LED transmitted illumination with adjustable intensity
- 100 W HBO fluorescence source for reflected fluorescence work
- Six-position fluorescence turret with UV shield
- Standard blue and green fluorescence filter configuration
- Optional violet, UV and red filter sets for broader fluorescence setup
Mechanics & Accessories
- Large 340 × 230 mm stage with coaxial X-Y movement 130 × 85 mm
- Included inserts for culture dishes, slides, multiwell and Terasaki plates
- Coaxial coarse and fine focusing with 1 µm precision
- Tilting condenser arm for easier access to larger sample vessels
- 1.5× lens changer enables intermediate magnifications without refocusing
- Optional digital camera, C-mount adapters and SLR photo accessories
Typical applications
- Cell culture and live sample observation in inverted configuration
- Routine bright field and fluorescence laboratory microscopy
- Phase contrast observation of transparent biological samples
- Multiwell plate, dish and slide based life science workflows
- Research imaging in university, institute and advanced laboratory settings
- DIC-ready applications for thicker specimens with pseudo three-dimensional contrast
- Microscopy work requiring optional digital capture or display integration
- Flexible fluorescence filter configuration for different dye sets
Configured package in this specification
| Base microscope set | FLUO-IN900 inverted microscope body, trinocular viewing head, HWF 10×/25 mm eyepieces, reversed sextuple nosepiece, long working distance phase objective set as listed in the overview, large mechanical stage and rotating N.A. 0.55 condenser |
| Illumination bundle | 10 W LED transmitted illumination with internal 100-240 V power supply, plus fluorescence module with 100 W HBO lamp |
| Fluorescence bundle | Six-position fluorescence attachment with UV shield and standard blue / green excitation filter configuration |
| Stage accessory bundle | Included inserts for Ø38 mm culture dish, Ø54 mm dish or 76 × 26 mm slides, multiwell plates and Terasaki plates |
| Configuration note | Several objectives, DIC components, camera modules, C-mounts and additional fluorescence filter sets are listed as optional accessories. Final supplied scope should follow quotation and order confirmation. |
Configuration note - FLUO-IN900
This technical sheet is organized around the uploaded FLUO-IN900 overview and accessory listing. The uploaded document combines base configuration details with optional modules, so this page separates core platform specifications from optional objectives, camera components, DIC parts and additional fluorescence filter sets. Final delivery scope should always be defined in the quotation.
This technical sheet is organized around the uploaded FLUO-IN900 overview and accessory listing. The uploaded document combines base configuration details with optional modules, so this page separates core platform specifications from optional objectives, camera components, DIC parts and additional fluorescence filter sets. Final delivery scope should always be defined in the quotation.
Frequently asked questions - FLUO-IN900
Is this an inverted microscope? Yes. The FLUO-IN900 is specified as an inverted fluorescence microscope for life science research.
Does it support fluorescence? Yes. The system uses a 100 W HBO mercury fluorescence source and a six-position fluorescence attachment with UV shield.
What observation modes are supported? The uploaded specification describes bright field, phase contrast, fluorescence, polarized light and DIC-ready capability.
Is digital imaging included? A 9.7" LCD with integrated 5 MP digital camera is listed as an accessory option rather than a mandatory standard item.
What sample holders are included? The stage is supplied with inserts for Ø38 mm dishes, Ø54 mm dishes or slides, multiwell plates and Terasaki plates.
Can the objective set be expanded? Yes. Additional phase, fluorescent and Plan-Apo objectives are listed among the optional accessories.
Is this an inverted microscope? Yes. The FLUO-IN900 is specified as an inverted fluorescence microscope for life science research.
Does it support fluorescence? Yes. The system uses a 100 W HBO mercury fluorescence source and a six-position fluorescence attachment with UV shield.
What observation modes are supported? The uploaded specification describes bright field, phase contrast, fluorescence, polarized light and DIC-ready capability.
Is digital imaging included? A 9.7" LCD with integrated 5 MP digital camera is listed as an accessory option rather than a mandatory standard item.
What sample holders are included? The stage is supplied with inserts for Ø38 mm dishes, Ø54 mm dishes or slides, multiwell plates and Terasaki plates.
Can the objective set be expanded? Yes. Additional phase, fluorescent and Plan-Apo objectives are listed among the optional accessories.
Please note: If this inverted fluorescence microscope configuration does not exactly match your application, contact our team. We can help with microscope configuration, fluorescence filter selection, stage insert selection, DIC accessory setup and digital imaging integration. Specifications subject to change without notice.
Contact & Support
COLO LabExperts
Polje ob Sotli 4
SI-3255, Slovenia
Support note: Contact us for inverted fluorescence microscope configuration, objective selection, fluorescence filter matching, DIC accessory planning and digital imaging setup.
Quick contact
Manufacturer & configuration notice:
Specifications in this document are based on the uploaded FLUO-IN900 technical catalog and arranged into the COLO.Science technical sheet format.
For procurement and tender purposes, the only valid and binding specification is the official COLO.Science quotation and the accompanying technical specification that is engineering-approved.
Specifications in this document are based on the uploaded FLUO-IN900 technical catalog and arranged into the COLO.Science technical sheet format.
For procurement and tender purposes, the only valid and binding specification is the official COLO.Science quotation and the accompanying technical specification that is engineering-approved.